The human brain is a complex organ that processes millions of neurons and transmits information through billions of synapses. Here, we perform a quantitative analysis of a fluorescent reporter of synaptic vesicle release in synapses to gain insights into the underlying patterns of synaptic transmission. In these experiments, dissociated rat hippocampal neurons expressing the reporter were electrically stimulated with field potential, and fluorescence signals were recorded. We observed a positive correlation between the resting intensity level after stimulation and resting intensity level before stimulation, peak value, and time interval to peak. These findings provide valuable insights into the response of individual synapses to electrical stimulation and uncover important features of synaptic transmission